Recombinant humaan UFSP1-eiwit

UFSP1 is similar to other ufm1-specific proteases. Studies in mouse determined that ufsp1 releases ufm1 (ubiquitin-fold modifier 1) from its bound conjugated complexes which also makes it into an active form. Because the human uFSP1 protein is shorter on the N-terminus and lacks a conserved Cys active site, it is predicted to be non-functional. Recombinant human uFSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inactive ufm1-specific protease 1, uFSP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.4 kDa (165aa) confirmed by MALDI-TOF

UFSP1, Inactive ufm1-specific protease 1, uFSP, ATGP1856-10 µg, ATGP1856-20 µg, ATGP1856-50 µg, ATGP1856-100 µg, ATGP1856-250 µg, ATGP1856-500 µg, ATGP1856-1 mg, ATGP1856-10, ATGP1856-20, ATGP1856-50, ATGP1856-100, ATGP1856-250, ATGP1856-500, ATGP1856-1

Wilson MD, Riemer C, et al. (2001) . Nucleic Acids Res. 29 (6) :1352-65

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Enzymes & Proteases

NP_001015072

Q6NVU6

Human

MGDKPPG FRGSRDWIGC VEASLCLAHF GGPQGRLCHV PRGVGLHGEL ERLYSHFAGG GGPVMVGGDA DARSKALLGV CVGSGTEAYV LVLDPHYWGT PKSPSELQAA GWVGWQEVSA AFDPNSFYNL CLTSLSSQQQ QRTLD