Recombinant humaan UGP2-eiwit

UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MguTP and forms uDP-glucose and MgPPi. In liver and muscle tissue, uDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to uDP-galactose which is then converted to lactose. Recombinant human uGP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

UDP-glucose pyrophosphorylase 2, UDP-glucose pyrophosphorylase 1, UTP--glucose-1-phosphate uridylyltransferase, UGP1, UGPP1, SVUGP2, UDPGP, UGPase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

59.3 kDa (531aa)

SVUGP 2, SVUGP2, UDP glucose pyrophosphorylase, UDP glucose pyrophosphorylase 1, UDP glucose pyrophosphorylase 2, UDPG, UDP-glucose pyrophosphorylase, UDP-glucose pyrophosphorylase 1, UDP-glucose pyrophosphorylase 2, UDPGP, UDPGP 2, UDPGP2, UGP 1, UGP 2, UGP1, UGP2, UGPase, UGPP 1, UGPP1, UTP glucose 1 phosphate uridylyltransferase, UTP-glucose-1-phosphate uridylyltransferase, UTP--glucose-1-phosphate uridylyltransferase, ATGP2865-10 µg, ATGP2865-20 µg, ATGP2865-50 µg, ATGP2865-100 µg, ATGP2865-250 µg, ATGP2865-500 µg, ATGP2865-1 mg, ATGP2865-010, ATGP2865-020, ATGP2865-050, ATGP2865-100, ATGP2865-250, ATGP2865-500, ATGP2865-01M

Fuhring, J., et al. (2013) Glycobiology 23 (4), 426-437

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol,1mM DTT

Metabolism

NP_006750.3

Q16851

Human

MSRFVQD LSKAMSQDGA SQFQEVIRQE LELSVKKELE KILTTASSHE FEHTKKDLDG FRKLFHRFLQ EKGPSVDWGK IQRPPEDSIQ PYEKIKARGL PDNISSVLNK LVVVKLNGGL GTSMGCKGPK SLIGVRNENT FLDLTVQQIE HLNKTYNTDV PLVLMNSFNT DEDTKKILQK YNHCRVKIYT FNQSRYPRIN KESLLPVAKD VSYSGENTEA WYPPGHGDIY ASFYNSGLLD TFIGEGKEYI FVSNIDNLGA TVDLYILNHL MNPPNGKRCE FVMEVTNKTR ADVKGGTLTQ YEGKLRLVEI AQVPKAHVDE FKSVSKFKIF NTNNLWISLA AVKRLQEQNA IDMEIIVNAK TLDGGLNVIQ LETAVGAAIK SFENSLGINV PRSRFLPVKT TSDLLLVMSN LYSLNAGSLT MSEKREFPTV PLVKLGSSFT KVQDYLRRFE SIPDMLELDH LTVSGDVTFG KNVSLKGTVI IIANHGDRID IPPGAVLENK IVSGNLRILD H