Recombinant humaan ULBP-1-eiwit

ULBP1 is ligand for the NKG2D receptor, together with at least uLBP2 and uLBP3. uLBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. In CMV infected cells, uLBP1 interacts with soluble CMV glycoprotein uL16. The interaction with uL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. uL16 also causes uLBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. Recombinant human uLBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

UL16 binding protein 1, ALCAN-beta, NKG2D ligand 1, N2DL-1, NKG2DL1, Retinoic acid early transcript 1I, RAET1I

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25 kDa (216aa) confirmed by MALDI-TOF

ULBP1, NKG2D ligand 1, uL16 binding protein 1, RAET1I, N2DL-1, N2DL1, NKG2D ligand 1, ATGP1731-10 µg, ATGP1731-20 µg, ATGP1731-50 µg, ATGP1731-100 µg, ATGP1731-250 µg, ATGP1731-500 µg, ATGP1731-1 mg, ATGP1731-10, ATGP1731-20, ATGP1731-50, ATGP1731-100, ATGP1731-250, ATGP1731-500, ATGP1731-1

Cerwenka A., et al. (2003) . Tissue Antigens. 61:335-343.; ; Bacon L., et al. (2004) . J Immunol. 173:1078-1084.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Immunology

NP_079494

Q9BZM6

Human

GWVDT HCLCYDFIIT PKSRPEPQWC EVQGLVDERP FLHYDCVNHK AKAFASLGKK VNVTKTWEEQ TETLRDVVDF LKGQLLDIQV ENLIPIEPLT LQARMSCEHE AHGHGRGSWQ FLFNGQKFLL FDSNNRKWTA LHPGAKKMTE KWEKNRDVTM FFQKISLGDC KMWLEEFLMY WEQMLDPTKP PSLAPG