Recombinant humaan ULBP-2-eiwit

ULBP2, also known as NKG2D ligand 2, belongs to the MHC class I family. This protein is ligand for the NKG2D receptor, together with at least uLBP1 and uLBP3. uLBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of uLBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein uL16. The interaction with uL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. uL16 also causes uLBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. Recombinant human uLBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

UL16 binding protein 2, ALCAN-alpha, NKG2D ligand 2, N2DL-2, NKG2DL2, Retinoic acid early transcript 1H, RAET1H, N2DL2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.3 kDa (216aa)

ULBP2, NKG2D ligand 2, N2DL2, RAET1H, ATGP1592-10 µg, ATGP1592-20 µg, ATGP1592-50 µg, ATGP1592-100 µg, ATGP1592-250 µg, ATGP1592-500 µg, ATGP1592-1 mg, ATGP1592-10, ATGP1592-20, ATGP1592-50, ATGP1592-100, ATGP1592-250, ATGP1592-500, ATGP1592-1

Cosman D., et al. (2001) Immunity. 14:123-133; ; Dunn C., et al. (2003) J. Exp. Med. 197:1427-1439

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2M urea, 0.2M NaCl, 2mM DTT

Immunology

NP_079493

Q9BZM5

Human

GRADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT FLHYDCGNKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLRDIQL ENYTPKEPLT LQARMSCEQK AEGHSSGSWQ FSFDGQIFLL FDSEKRMWTT VHPGARKMKE KWENDKVVAM SFHYFSMGDC IGWLEDFLMG MDSTLEPSAG APLAMS