Recombinant humaan ULBP-4/RAET1E eiwit

RAET1E, also known as NKG2D ligand 4 isoform 1, is a member of the RAET1/ULBP family of cell surface protein that function as ligands for NKG2D. This protein is abnormally expressed on most colon cancer and some other tumor cell lines and virus infected peripheral blood cells. It binds and co-stimulates NKG2D expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production. Also, it functions as a stress-induced ligand for NKG2D receptor. Recombinant human RAET1E, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Retinoic acid early transcript 1E, Lymphocyte effector toxicity activation ligand, NKG2D ligand 4, N2DL-4, NKG2DL4, RAE-1-like transcript 4, UL16-binding protein 4, LETAL, N2DL4, ULBP4

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

49.6 kDa (437aa)

ULBP4, RAET1E, NKG2D ligand 4 isoform 1, RAET1E, bA350J20.7, LETAL, N2DL-4, NKG2DL4, RAET1E2, RL-4, ULBP4, ATGP3656-10 µg, ATGP3656-20 µg, ATGP3656-50 µg, ATGP3656-100 µg, ATGP3656-250 µg, ATGP3656-500 µg, ATGP3656-1 mg, ATGP3656-10, ATGP3656-20, ATGP3656-50, ATGP3656-100, ATGP3656-250, ATGP3656-500, ATGP3656-1

Chalupny NJ., et al, (2003) Biochem. Biophys. Res. Commun. 305:129-135.; ; Kong Y., et al, (2009) Blood 114:310-317.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_631904

Q8TD07

Human

Ala

ADPHSLCFNF TIKSLSRPGQ PWCEAQVFLN KNLFLQYNSD NNMVKPLGLL GKKVYATSTW GELTQTLGEV GRDLRMLLCD IKPQIKTSDP STLQVEMFCQ REAERCTGAS WQFATNGEKS LLFDAMNMTW TVINHEASKI KETWKKDRGL EKYFRKLSKG DCDHWLREFL GHWEAMPEPT VSPVNASDIH WSSSSLPDVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH