Recombinant humaan UNC119B eiwit

UCN119B (Protein unc-119 homolog B) belongs to the PDE6D/unc-119 family. This protein is enriched in the photoreceptors of the retina. It shares strong homology with the C. elegans unc119 protein and it can functionally complement the C. elegans unc119 mutation gene. The mammalian multisubunit photoreceptor cGMP phosphodiesterase PDE alpha beta gamma 2 (PDE6 family) is a peripherally membrane-associated enzyme. Recombinant human uNC119B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein unc-119 homolog B, POC7B, unc-119 lipid binding chaperone B, POC7 centriolar protein homolog B

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.3 kDa (271aa) confirmed by MALDI-TOF

UNC119B, Protein unc-119 homolog B, POC7B, ATGP1398-10 µg, ATGP1398-20 µg, ATGP1398-50 µg, ATGP1398-100 µg, ATGP1398-250 µg, ATGP1398-500 µg, ATGP1398-1 mg, ATGP1398-10, ATGP1398-20, ATGP1398-50, ATGP1398-100, ATGP1398-250, ATGP1398-500, ATGP1398-1

Li N, et al. (1998) Genomics. 49 (1) :76-82.; ; Ota T., et al. (2004) Nat. Genet. 36:40-45

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Enzymes & Proteases

NP_001074002

A6NIH7

Human

MSGSNPKAAA AASAAGPGGL VAGKEEKKKA GGGVLNRLKA RRQAPHHAAD DGVGAAVTEQ ELLALDTIRP EHVLRLSRVT ENYLCKPEDN IYSIDFTRFK IRDLETGTVL FEIAKPCVSD QEEDEEEGGG DVDISAGRFV RYQFTPAFLR LRTVGATVEF TVGDKPVSNF RMIERHYFRE HLLKNFDFDF GFCIPSSRNT CEHIYEFPQL SEDVIRLMIE NPYETRSDSF YFVDNKLIMH NKADYAYNGG Q