Recombinant humaan UPK3A-eiwit

UPK3A belongs to the uroplakin-3 family. uroplakins (uP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (uPII, uPIIIa, and uPIIIb) or four times (uPIa and uPIb; both belonging to the 'tetraspanin' family) . All have large luminal/extracellular domains, but only uPIIIa and uPIIIb have significant cytoplasmic portions in their C-termini. uPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia Recombinant human uPK3A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Uroplakin-3a, uPK3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.1 kDa (214aa) confirmed by MALDI-TOF

UPK3A, uroplakin-3a, uPK3, ATGP1304-10 µg, ATGP1304-20 µg, ATGP1304-50 µg, ATGP1304-100 µg, ATGP1304-250 µg, ATGP1304-500 µg, ATGP1304-1 mg, ATGP1304-10, ATGP1304-20, ATGP1304-50, ATGP1304-100, ATGP1304-250, ATGP1304-500, ATGP1304-1

Shapiro, E., et al. (2000) J. urol. 164: 1048-1051.; ; Wu, X.R., et al. (1994) J. Biol. Chem. 269:13716-13724.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150 mM NaCl

Cancer

BAA31460.1

O75631

Human

VNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG