Recombinant humaan UPM1-eiwit

UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation. It localizes primarily to the nucleus, but is also present in diffuse amounts in the cytoplasm. This protein is expressed in a variety of tissues, including kidney, brain, heart, liver and lung. Recombinant human UFM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-fold modifier 1, bA131P10.1, BM-002, C13orf20

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

11.1 kDa (103aa) confirmed by MALDI-TOF

UFM1, ubiquitin-fold modifier 1, bA131P10.1, BM-002, C13orf20, ATGP0940-10 µg, ATGP0940-20 µg, ATGP0940-50 µg, ATGP0940-100 µg, ATGP0940-250 µg, ATGP0940-500 µg, ATGP0940-1 mg, ATGP0940-10, ATGP0940-20, ATGP0940-50, ATGP0940-100, ATGP0940-250, ATGP0940-500, ATGP0940-1

Sasakawa H., et al. (2006) Biochem Biophys Res Commun. 343 (1) :21-6. ; ; Komatsu M., et al. (2004) EMBO J. 23 (9) :1977-86.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Ubiquitination

NP_057701.1

P61960

Human

MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTAGNVFLKH GSELRIIPRD RVG