Recombinant humaan UPP1-eiwit

Uridine phosphorylase 1, also known as uPP1 catalyses the reversible phosphorolysis of uridine to uracil. The reaction products are then utilized as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis. The expression levels and the enzymatic activity of uPP1 are higher in human solid tumors than in adjacent normal tissues. In addition, uPP1 controls the homeostatic regulation of uridine concentration in plasma and tissues and plays a role in the intracellular activation of 5-fluorouracil. Recombinant human uPP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Uridine phosphorylase 1, uP, uPASE, uPP, urdPase 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36 kDa (330aa) confirmed by MALDI-TOF

UPP1, uridine phosphorylase 1, uP, uPASE, uPP, urdPase 1, ATGP0707-10 µg, ATGP0707-20 µg, ATGP0707-50 µg, ATGP0707-100 µg, ATGP0707-250 µg, ATGP0707-500 µg, ATGP0707-1 mg, ATGP0707-10, ATGP0707-20, ATGP0707-50, ATGP0707-100, ATGP0707-250, ATGP0707-500, ATGP0707-1

Kanzaki A., et al. (2002) Int J Cancer. 97 (5) :631-5.; ; Russll RL., et al. (2001) J Biol Chem. 276 (16) :13302-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 200 mM NaCl

Cancer

NP_853628

Q16831

Human

MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS YFIKKKLSKA