Recombinant humaan UPRT-eiwit

UPRT is a uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (uMP) . This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. Recombinant human uPRT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Uracil phosphoribosyltransferase homolog, uracil phosphoribosyltransferase (FuR1) homolog (S.cerevisiae), FuR1, RP11-311P8.3, uPP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.2 kDa (332aa) confirmed by MALDI-TOF

UPRT, uracil phosphoribosyltransferase homolog, uracil phosphoribosyltransferase (FuR1) homolog (S.cerevisiae), FuR1, RP11-311P8.3, uPP, ATGP2309-10 µg, ATGP2309-20 µg, ATGP2309-50 µg, ATGP2309-100 µg, ATGP2309-250 µg, ATGP2309-500 µg, ATGP2309-1 mg, ATGP2309-10, ATGP2309-20, ATGP2309-50, ATGP2309-100, ATGP2309-250, ATGP2309-500, ATGP2309-1

T. Selwood and E. K. Jaffe. (2011) Arch. Biochem. Biophys. 519 (2) : 131-43.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_659489

Q96BW1

Human

MATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSG ACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPY KECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTV IEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD