Recombinant humaan URM1-eiwit

URM1 is ubiquitin-related modifier 1 homolog protein that primarily functions in the post-translational modification of proteins by way of the urmylation pathway. In studies with Saccharomyces cerevisiae, it has been found that urm1 covalently binds to its E1 activating enzyme, uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1) . It is hypothesized that this complex may then play a role in the oxidative-stress response in mammals. Recombinant human uRM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-related modifier 1 homolog, C9orf74, MGC2668, RP11-339B21.4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

13.5 kDa (121aa) confirmed by MALDI-TOF

URM1, ubiquitin-related modifier 1 homolog, C9orf74, MGC2668, RP11-339B21.4, ATGP0994-10 µg, ATGP0994-20 µg, ATGP0994-50 µg, ATGP0994-100 µg, ATGP0994-250 µg, ATGP0994-500 µg, ATGP0994-1 mg, ATGP0994-10, ATGP0994-20, ATGP0994-50, ATGP0994-100, ATGP0994-250, ATGP0994-500, ATGP0994-1

Xu J., et al. (2006) Proc Natl Acad Sci u S A. 103 (31) :11625-30. ; ; Schlieker CD., et al. (2008) Proc Natl Acad Sci u S A. 105 (47) :18255-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Ubiquitination

NP_112176

Q9BTM9

Human

MAAPLSVEVE FGGGAELLFD GIKKHRVTLP GQEEPWDIRN LLIWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SVLFISTLHG G