Recombinant humaan UROD-eiwit

UROD is the fifth enzyme in the human heme biosynthetic pathway and is responsible for the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are responsible for three autosomal disorders in humans: familial porphyria cutanea tarda (f-PCT), sporadic porphyria cutanea tarda (s-PCT) and hepatoerythropoietic porphyria (HEP) . Recombinant human UROD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Uroporphyrinogen decarboxylase, PCT, uroporphyrinogen decarboxylase, uPD, uRO D, uRO-D

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

43.0 kDa (387aa) confirmed by MALDI-TOF

UROD, uroporphyrinogen decarboxylase, PCT, uroporphyrinogen decarboxylase, uPD, uRO D, uRO-D, ATGP0612-10 µg, ATGP0612-20 µg, ATGP0612-50 µg, ATGP0612-100 µg, ATGP0612-250 µg, ATGP0612-500 µg, ATGP0612-1 mg, ATGP0612-10, ATGP0612-20, ATGP0612-50, ATGP0612-100, ATGP0612-250, ATGP0612-500, ATGP0612-1

Lewis CA Jr., et al. (2008) Proc Natl Acad Sci u S A. 105 (45) :17328-33. ; ; Garey JR., et al. (1989) Blood. 73 (4) :892-5. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, and 1mM EDTA

Enzymes & Proteases

AAH01778

P06132

Human

MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQVNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN