Recombinant humaan UROS-eiwit

UROS, also known as uroporphyrinogen III synthase, is an enzyme involved in the fourth step of porphyrin metabolism, involved in the conversion of hydroxymethyl bilane into uroporphyrinogen III. Defects in this protein can cause molecular lesions that lead to the autosomal recessive Gunther disease, also known as congenital erythropoietic porphyria (CEP) . Recombinant human uROS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Uroporphyrinogen-III synthase, uroporphyrinogen III synthase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.7 kDa (285aa) confirmed by MALDI-TOF

UROS, uroporphyrinogen-III synthase, uroporphyrinogen III synthase, ATGP1066-10 µg, ATGP1066-20 µg, ATGP1066-50 µg, ATGP1066-100 µg, ATGP1066-250 µg, ATGP1066-500 µg, ATGP1066-1 mg, ATGP1066-10, ATGP1066-20, ATGP1066-50, ATGP1066-100, ATGP1066-250, ATGP1066-500, ATGP1066-1

Geronimi F. et al. (2003) J. Mol. Med. 81: 310-320.; ; Omata Y. et al. (2004) J. BioChem. 136: 211-220.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_000366

P10746

Human

MKVLLLKDAK EDDCGQDPYI RELGLYGLEA TLIPVLSFEF LSLPSFSEKL SHPEDYGGLI FTSPRAVEAA ELCLEQNNKT EVWERSLKEK WNAKSVYVVG NATASLVSKI GLDTEGETCG NAEKLAEYIC SRESSALPLL FPCGNLKREI LPKALKDKGI AMESITVYQT VAHPGIQGNL NSYYSQQGVP ASITFFSPSG LTYSLKHIQE LSGDNIDQIK FAAIGPTTAR ALAAQGLPVS CTAESPTPQA LATGIRKALQ PHGCC