Recombinant humaan USF1-eiwit

USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. USF1 can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL) . Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. Recombinant human USF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Upstream transcription factor 1, Upstream stimulatory factor 1, uEF, MLTFI, MLTF, Major late transcription factor 1, HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.9 kDa (333aa) confirmed by MALDI-TOF

USF1, upstream transcription factor 1, bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, uEF, ATGP2447-10 µg, ATGP2447-20 µg, ATGP2447-50 µg, ATGP2447-100 µg, ATGP2447-250 µg, ATGP2447-500 µg, ATGP2447-1 mg, ATGP2447-10, ATGP2447-20, ATGP2447-50, ATGP2447-100, ATGP2447-250, ATGP2447-500, ATGP2447-1

Rahaus M., et al. (2003) J. Gen. Virol. 84:2957-2967; ; Pajukanta P., et al. (2004) Nat. Genet. 36:371-376;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_001263302

P22415

Human

MKGQQKT AETEEGTVQI QEGAVATGED PTSVAIASIQ SAATFPDPNV KYVFRTENGG QVMYRVIQVS EGQLDGQTEG TGAISGYPAT QSMTQAVIQG AFTSDDAVDT EGTAAETHYT YFPSTAVGDG AGGTTSGSTA AVVTTQGSEA LLGQATPPGT GQFFVMMSPQ EVLQGGSQRS IAPRTHPYSP KSEAPRTTRD EKRRAQHNEV ERRRRDKINN WIVQLSKIIP DCSMESTKSG QSKGGILSKA CDYIQELRQS NHRLSEELQG LDQLQLDNDV LRQQVEDLKN KNLLLRAQLR HHGLEVVIKN DSN