Recombinant humaan USP15-eiwit

USP15 is a member of the ubiquitin specific protease (uSP) family of deubiquitinating enzymes. uSP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. Recombinant human uSP15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin carboxyl-terminal hydrolase 15 isoform 3, uNPH-2, uNPH4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.5 kDa (258aa) confirmed by MALDI-TOF

USP15, ubiquitin carboxyl-terminal hydrolase 15 isoform 3, uNPH-2, uNPH4, ATGP2237-10 µg, ATGP2237-20 µg, ATGP2237-50 µg, ATGP2237-100 µg, ATGP2237-250 µg, ATGP2237-500 µg, ATGP2237-1 mg, ATGP2237-10, ATGP2237-20, ATGP2237-50, ATGP2237-100, ATGP2237-250, ATGP2237-500, ATGP2237-1

Imboden, M., et al. (2012) J. Allergy Clin. Immunol. 129 (5), 1218-1228; ; Eichhorn, P.J., et al. (2012) Nat. Med. 18 (3), 429-435

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001239008

Q9Y4E8

Human

MAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC