Recombinant humaan USP46/UAF1-eiwit

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. uSP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. Recombinant human uSP46 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin carboxyl-terminal hydrolase 46 isoform 1, Deubiquitinating enzyme 46, FLJ12552, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin thioesterase 46, ubiquitin-specific-processing protease 46

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BRADFORD assay)

> 90% by SDS-PAGE

44.8 kDa (389aa) confirmed by MALDI-TOF

USP46, UAF1, ubiquitin carboxyl-terminal hydrolase 46 isoform 1, Deubiquitinating enzyme 46, FLJ12552, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin thioesterase 46, ubiquitin-specific-processing protease 46, ATGP2553-10 µg, ATGP2553-20 µg, ATGP2553-50 µg, ATGP2553-100 µg, ATGP2553-250 µg, ATGP2553-500 µg, ATGP2553-1 mg, ATGP2553-10, ATGP2553-20, ATGP2553-50, ATGP2553-100, ATGP2553-250, ATGP2553-500, ATGP2553-1

Fukuo, Y., et al. (2011) J Affect Disord 133 (1-2), 150-157; ; Zhang, W., et al. (2011) PLoS ONE 6 (10), E26297;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_073743.2

P62068

Human

MTVRNIA SICNMGTNAS ALEKDIGPEQ FPINEHYFGL VNFGNTCYCN SVLQALYFCR PFRENVLAYK AQQKKKENLL TCLADLFHSI ATQKKKVGVI PPKKFISRLR KENDLFDNYM QQDAHEFLNY LLNTIADILQ EEKKQEKQNG KLKNGNMNEP AENNKPELTW VHEIFQGTLT NETRCLNCET VSSKDEDFLD LSVDVEQNTS ITHCLRDFSN TETLCSEQKY YCETCCSKQE AQKRMRVKKL PMILALHLKR FKYMEQLHRY TKLSYRVVFP LELRLFNTSS DAVNLDRMYD LVAVVVHCGS GPNRGHYITI VKSHGFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRE