Recombinant humaan VAMP-4-eiwit

VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Vesicle-associated membrane protein 4, VAMP24, VAMP 4, Vesicle associated membrane protein 4 isoform CRA a, Vesicle associated membrane protein 4 isoform CRA b

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

14.5 kDa (123aa) confirmed by MALDI-TOF

Vesicle-associated membrane protein 4, Vesicle associated membrane protein 4 isoform CRA b, Vesicle associated membrane protein 4 isoform CRA a, Vesicle associated membrane protein 4, VAMP-4 protein, VAMP4 protein, VAMP-4, VAMP4, VAMP-24, VAMP24, VAMP 4 protein, VAMP 4, VAMP 24, isoform CRA b, isoform CRA a, ATGP0360-10 µg, ATGP0360-20 µg, ATGP0360-50 µg, ATGP0360-100 µg, ATGP0360-250 µg, ATGP0360-500 µg, ATGP0360-1 mg, ATGP0360-010, ATGP0360-020, ATGP0360-050, ATGP0360-100, ATGP0360-250, ATGP0360-500, ATGP0360-01M

Tran TH., et al. (2007) J Cell Sci. 120:1028-41.; ; Steegmaier M., et al. (1999) Mol Biol Cell. 10 (6) :1957-72.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Neuroscience

NP_003753

O75379

Human

MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH