Recombinant humaan VAP-1/AOC3-eiwit

VAP-1, also known as membrane primary amine oxidase isoform 1, a copper amine oxidase with a topaquinone cofactor. It is a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. This protein plays a role in adipogenesis. It catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. Recombinant human VAP-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

VAP-1, AOC3, HPAO, VAP1, Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

82.8 kDa (746aa)

VAP1, AOC3, ATGP3924-10 µg, ATGP3924-20 µg, ATGP3924-50 µg, ATGP3924-100 µg, ATGP3924-250 µg, ATGP3924-500 µg, ATGP3924-1 mg, ATGP3924-10, ATGP3924-20, ATGP3924-50, ATGP3924-100, ATGP3924-250, ATGP3924-500, ATGP3924-1

Hsia LT., et al. (2016) Proc Natl Acad Sci U S A. 113:E2162-71; ; Trivedi PJ., et al. (2018) Gut. 67:1135-1145

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_003725

Q16853

Human

Ala

GRGGDGG EPSQLPHCPS VSPSAQPWTH PGQSQLFADL SREELTAVMR FLTQRLGPGL VDAAQARPSD NCVFSVELQL PPKAAALAHL DRGSPPPARE ALAIVFFGRQ PQPNVSELVV GPLPHPSYMR DVTVERHGGP LPYHRRPVLF QEYLDIDQMI FNRELPQASG LLHHCCFYKH RGRNLVTMTT APRGLQSGDR ATWFGLYYNI SGAGFFLHHV GLELLVNHKA LDPARWTIQK VFYQGRYYDS LAQLEAQFEA GLVNVVLIPD NGTGGSWSLK SPVPPGPAPP LQFYPQGPRF SVQGSRVASS LWTFSFGLGA FSGPRIFDVR FQGERLVYEI SLQEALAIYG GNSPAAMTTR YVDGGFGMGK YTTPLTRGVD CPYLATYVDW HFLLESQAPK TIRDAFCVFE QNQGLPLRRH HSDLYSHYFG GLAETVLVVR SMSTLLNYDY VWDTVFHPSG AIEIRFYATG YISSAFLFGA TGKYGNQVSE HTLGTVHTHS AHFKVDLDVA GLENWVWAED MVFVPMAVPW SPEHQLQRLQ VTRKLLEMEE QAAFLVGSAT PRYLYLASNH SNKWGHPRGY RIQMLSFAGE PLPQNSSMAR GFSWERYQLA VTQRKEEEPS SSSVFNQNDP WAPTVDFSDF INNETIAGKD LVAWVTAGFL HIPHAEDIPN TVTVGNGVGF FLRPYNFFDE DPSFYSADSI YFRGDQDAGA CEVNPLACLP QAAACAPDLP AFSHGGFSHN