Recombinant humaan VAP-B-eiwit

VAPB, also known as vesicle-associated membrane protein (VAMP) -associated protein B, is a type IV transmembrane protein and member of the VAP family of proteins. This protein may play a role in vesicle trafficking. It is found in plasma and intracellular vesicle membranes as a homodimer and heterodimer with VAPA, and interacts with VAMP1 and VAMP2. Defects in VAPB are a cause of amyotrophic lateral sclerosis type 8 and spinal muscular atrophy autosomal dominant Finkel type. Recombinant human VAPB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Vesicle-associated membrane protein-associated protein B/C, ALS8, VAMP-B, VAMP-C, VAP-B, VAP-C

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.1 kDa (242aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

VAP-B, Vesicle-associated membrane protein-associated protein B/C, ALS8, VAMP-B, VAMP-C, VAP-B, VAP-C, ATGP0800-10 µg, ATGP0800-20 µg, ATGP0800-50 µg, ATGP0800-100 µg, ATGP0800-250 µg, ATGP0800-500 µg, ATGP0800-1 mg, ATGP0800-10, ATGP0800-20, ATGP0800-50, ATGP0800-100, ATGP0800-250, ATGP0800-500, ATGP0800-1

Nishimura AL, et al. (2004), Am J Hum Genet., 75 (5) :822-31.; ; Hamamoto I., et al. (2005) . J Virol., 79 (21) :13473-82.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Neuroscience

NP_004729

O95292

Human

MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGI IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS DMEAVWKEAK PEDLMDSKLR CVFELPAEND KPHDVEINKI ISTTASKTET PIVSKSLSSS LDDTEVKKVM EECKRLQGEV QRLREENKQF KEEDGLRMRK TVQSNSPISA LAPTGKEEGL ST