Recombinant humaan VASP-eiwit

Vasodilator-stimulated phosphoprotein, also known as VASP, is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. Recombinant human VASP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Vasodilator-stimulated phosphoprotein

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37.5 kDa (363aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

VASP, Vasodilator-stimulated phosphoprotein, ATGP1003-10 µg, ATGP1003-20 µg, ATGP1003-50 µg, ATGP1003-100 µg, ATGP1003-250 µg, ATGP1003-500 µg, ATGP1003-1 mg, ATGP1003-10, ATGP1003-20, ATGP1003-50, ATGP1003-100, ATGP1003-250, ATGP1003-500, ATGP1003-1

Laurent V., et al. (1999) J. Cell Biol. 144:1245-1258; ; Barzik M., et al. (2005) J. Biol. Chem. 280:28653-28662

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl, 0.1mM PMSF

Cytoskeleton

NP_003361

P50552

Human

MGSSHHHHHH SSGLVPRGSH MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEGGGPPP PPALPTWSVP NGPSPEEVEQ QKRQQPGPSE HIERRVSNAG GPPAPPAGGP PPPPGPPPPP GPPPPPGLPP SGVPAAAHGA GGGPPPAPPL PAAQGPGGGG AGAPGLAAAI AGAKLRKVSK QEEASGGPTA PKAESGRSGG GGLMEEMNAM LARRRKATQV GEKTPKDESA NQEEPEARVP AQSESVRRPW EKNSTTLPRM KSSSSVTTSE TQPCTPSSSD YSD