Recombinant humaan Vav-1-eiwit

Proto-oncogene vav isoform 1, also known as VAV1, is a member of the VAV gene family. The VAV1 proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. In addition, VAV1 contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of VAV1 is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. Recombinant human VAV1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proto-oncogene vav isoform 1, Proto-oncogene vav isoform 1, VAV, Proto-oncogene vav, vav 1 guanine nucleotide exchange factor, vav 1 oncogene

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.8 kDa (400aa) confirmed by MALDI-TOF

Vav1, Proto-oncogene vav isoform 1, Proto-oncogene vav isoform 1, VAV, Proto-oncogene vav, vav 1 guanine nucleotide exchange factor, vav 1 oncogene, ATGP2424-10 µg, ATGP2424-20 µg, ATGP2424-50 µg, ATGP2424-100 µg, ATGP2424-250 µg, ATGP2424-500 µg, ATGP2424-1 mg, ATGP2424-10, ATGP2424-20, ATGP2424-50, ATGP2424-100, ATGP2424-250, ATGP2424-500, ATGP2424-1

Bustelo X R., et al. (1992) Nature. 356:68-71.; ; Katzav S., et al. (1989) EMBO J. 8:2283-2290.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT

Cancer

NP_005419

P15498

Human

MTEYDKR CCCLREIQQT EEKYTDTLGS IQQHFLKPLQ RFLKPQDIEI IFINIEDLLR VHTHFLKEMK EALGTPGAAN LYQVFIKYKE RFLVYGRYCS QVESASKHLD RVAAAREDVQ MKLEECSQRA NNGRFTLRDL LMVPMQRVLK YHLLLQELVK HTQEAMEKEN LRLALDAMRD LAQCVNEVKR DNETLRQITN FQLSIENLDQ SLAHYGRPKI DGELKITSVE RRSKMDRYAF LLDKALLICK RRGDSYDLKD FVNLHSFQVR DDSSGDRDNK KWSHMFLLIE DQGAQGYELF FKTRELKKKW MEQFEMAISN IYPENATANG HDFQMFSFEE TTSCKACQML LRGTFYQGYR CHRCRASAHK ECLGRVPPCG