Recombinant humaan VBP1-eiwit

Prefoldin subunit 3, also known as VBP1, belongs to the prefoldin subunit alpha family. VBP1 interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. Recombinant human VBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prefoldin subunit 3, PFD3, PFDN3, VBP-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25 kDa (220aa) confirmed by MALDI-TOF

VBP1, Prefoldin subunit 3, PFD3, PFDN3, VBP-1, ATGP1861-10 µg, ATGP1861-20 µg, ATGP1861-50 µg, ATGP1861-100 µg, ATGP1861-250 µg, ATGP1861-500 µg, ATGP1861-1 mg, ATGP1861-10, ATGP1861-20, ATGP1861-50, ATGP1861-100, ATGP1861-250, ATGP1861-500, ATGP1861-1

Kim S Y., et al. (2008) BMB Rep. 41 (2) :158-63.; ; Mousnier A., et al. (2007) Proc Natl Acad Sci uSA. 201 (34) :13612-20.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Heat Shock Proteins

NP_003363

P61758

Human

MAAVKDS CGKGEMATGN GRRLHLGIPE AVFVEDVDSF MKQPGNETAD TVLKKLDEQY QKYKFMELNL AQKKRRLKGQ IPEIKQTLEI LKYMQKKKES TNSMETRFLL ADNLYCKASV PPTDKVCLWL GANVMLEYDI DEAQALLEKN LSTATKNLDS LEEDLDFLRD QFTTTEVNMA RVYNWDVKRR NKDDSTKNKA