Recombinant humaan VCAM-1/CD106-eiwit

VCAM1, also known as vascular cell adhesion protein 1 isoform, a member of the immunoglobulin superfamily. It is a cell surface protein expressed by activated endothelial cells and certain leukocytes. VCAM1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection and asthmatic responses. During the inflammatory adhesion mechanism, activated integrins that rolling leukocytes and attach them firmly to the vascular endothelium. Recombinant human VCAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10 µg/mL. This effect is more to 70%.

Vascular cell adhesion protein 1 isoform, VCAM1, CD106, INCAM-100

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10 µg/mL. This effect is more to 70%.

75.6 kDa (685aa)

VCAM1, CD106, Vascular cell adhesion protein 1 isoform, VCAM1, CD106, INCAM-100, ATGP3468-10 µg, ATGP3468-20 µg, ATGP3468-50 µg, ATGP3468-100 µg, ATGP3468-250 µg, ATGP3468-500 µg, ATGP3468-1 mg, ATGP3468-10, ATGP3468-20, ATGP3468-50, ATGP3468-100, ATGP3468-250, ATGP3468-500, ATGP3468-1

Cybulsky MI., et al. (1991) Am. J. Pathol. 138:815-820.; ; Preiss DJ., et al. (2007) Int. J. Clin. Pract 61:697-701.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001069

P19320

Human

Ala

FKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPE