Recombinant humaan VEGF 121/VEGFA-eiwit

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.

Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/mL.

14.2 kDa (122aa) confirmed by MALDI-TOF

VEGF121, ATGP0282-10 µg, ATGP0282-20 µg, ATGP0282-50 µg, ATGP0282-100 µg, ATGP0282-250 µg, ATGP0282-500 µg, ATGP0282-1 mg, ATGP0282-10, ATGP0282-20, ATGP0282-50, ATGP0282-100, ATGP0282-250, ATGP0282-500, ATGP0282-1

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1) :16-24.; ; Neufeld G., et al. (1999) FASEB J. 13 (1) :9-22.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol

Hormones & Cytokines

NP_001020541.2

P15692

Human

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR