Recombinant humaan VEGF 165 eiwit

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

21.4 kDa (186aa) conformed by MALDI-TOF

VEGF165, ATGP1248-10 µg, ATGP1248-20 µg, ATGP1248-50 µg, ATGP1248-100 µg, ATGP1248-250 µg, ATGP1248-500 µg, ATGP1248-1 mg, ATGP1248-10, ATGP1248-20, ATGP1248-50, ATGP1248-100, ATGP1248-250, ATGP1248-500, ATGP1248-1

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1) :16-24. ; ; Neufeld G., et al. (1999) FASEB J. 13 (1) :9-22.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) 5mM DTT, 50 % glycerol, 200 mM NaCl, 2mM EDTA

Hormones & Cytokines

NP_001020539.2

P15692

Human

APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR