Recombinant humaan VEGF 165 eiwit

Vascular endothelial growth factor A, also known as VEGF, is growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. This protein binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Recombinant human VEGF165 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

Vascular endothelial growth factor A, Vascular endothelial growth factor A, MVCD1, VEGF, VPF

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

19.9 kDa (171aa)

VEGF165, Vascular endothelial growth factor A, Vascular endothelial growth factor A, MVCD1, VEGF, VPF, ATGP2873-10 µg, ATGP2873-20 µg, ATGP2873-50 µg, ATGP2873-100 µg, ATGP2873-250 µg, ATGP2873-500 µg, ATGP2873-1 mg, ATGP2873-10, ATGP2873-20, ATGP2873-50, ATGP2873-100, ATGP2873-250, ATGP2873-500, ATGP2873-1

Woolard J., et al. (2004) Cancer Res. 64:7822-7835.; ; Dixelius J., et al. (2006) Cancer Res. 66:2089-2097.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 1mM DTT, 0.1mM PMSF

Hormones & Cytokines

NP_001165097

P15692

Human