Recombinant humaan Visinine-achtig eiwit-1/VILIP-eiwit

Visinin-like protein-1, also known as VILIP-1, belongs to a family of neuronal calcium sensor proteins. VILIP-1 is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Also, it is expressed in pancreatic beta-cells. VILIP-1 elevation enhances insulin secretion in cAMP-associated manner. Down-regulation of VILIP-1 decreased cAMP accumulation but increased insulin gene transcription. Recombinant human Visinin-like protein-1 was expressed in E. coli and purified by using conventional chromatography.

VILIP, VILIP-1, VLP-1, HLP3, VSNL1, Hippocalcin like protein 3, HLP 3, HPCAL 3, HPCAL3, HuVISL1, Neurocalcin alpha, NVL 1, NVL1, VILIP, Visinin.Visinin like 1, Visinin like protein 1.VISL 1.

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

22.1 kDa (191aa) confirmed by MALDI-TOF

Visinin-like protein1, VILIP, VILIP-1, VLP-1, HLP3, VSNL1, Hippocalcin like protein 3, HLP 3, HPCAL 3, HPCAL3, HuVISL1, Neurocalcin alpha, NVL 1, NVL1, VILIP, Visinin.Visinin like 1, Visinin like protein 1.VISL 1., ATGP0384-10 µg, ATGP0384-20 µg, ATGP0384-50 µg, ATGP0384-100 µg, ATGP0384-250 µg, ATGP0384-500 µg, ATGP0384-1 mg, ATGP0384-10, ATGP0384-20, ATGP0384-50, ATGP0384-100, ATGP0384-250, ATGP0384-500, ATGP0384-1

Lenz SE., et al. (1996) Biochem Biophys Res Commun. 225 (3) :1078-83.; ; Braunewell KH., et al. (1997) J Neurochem. 68 (5) :2129-39.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol

Neuroscience

NP_003376

P62760

Human

MGKQNSKLAP EVMEDLVKST EFNEHELKQW YKGFLKDCPS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPS IVLLLQCDIQ K