Recombinant humaan VOPP1-eiwit

Vesicular, overexpressed in cancer, prosurvival protein 1 (VOPP1), also known as Glioblastoma-amplified secreted protein (GASP), has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. VOPP1 is a key regulator of NF-kappaB signaling, and that high-level, amplification-mediated VOPP1 expression, such as that occurring in tumors with amplified EGFR, could contribute to resistance to apoptosis. Recombinant human VOPP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Vesicular overexpressed in cancer prosurvival protein 1, Vesicular, overexpressed in cancer, prosurvival protein 1, ECOP, GASP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

12 kDa (112aa) confirmed by MALDI-TOF

VOPP1, Vesicular overexpressed in cancer prosurvival protein 1, Vesicular, overexpressed in cancer, prosurvival protein 1, ECOP, GASP, ATGP1076-10 µg, ATGP1076-20 µg, ATGP1076-50 µg, ATGP1076-100 µg, ATGP1076-250 µg, ATGP1076-500 µg, ATGP1076-1 mg, ATGP1076-10, ATGP1076-20, ATGP1076-50, ATGP1076-100, ATGP1076-250, ATGP1076-500, ATGP1076-1

Park S., et al. (2005) Oncogene. 24 (15) :2495-502.; ; Barse A., et al. (2010) J Mol Histol. 41 (2) :153-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Cancer

NP_110423.3

Q96AW1

Human

RRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK