Recombinant humaan VPS22/SNF8-eiwit

SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Recombinant human SNF8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SNF8 subunit of ESCRT-II, Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, EAP30, ESCRT-II complex subunit VPS22, hVps22, VPS22, Dot3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.4 kDa (282aa) confirmed by MALDI-TOF

SNF8, Vacuolar-sorting protein SNF8, Dot3, EAP30, VPS22, ATGP1594-10 µg, ATGP1594-20 µg, ATGP1594-50 µg, ATGP1594-100 µg, ATGP1594-250 µg, ATGP1594-500 µg, ATGP1594-1 mg, ATGP1594-10, ATGP1594-20, ATGP1594-50, ATGP1594-100, ATGP1594-250, ATGP1594-500, ATGP1594-1

Maleroed L., et al. (2007) Traffic 8:1617-1629; ; Raiborg C., et al. (2008) Exp. Cell Res. 314:801-813

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 2mM DTT, 200 mM NaCl

Epigenomics (Transcription & Translation)

NP_009172.2

Q96H20

Human

HRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP