Recombinant humaan VPS24/CHMP3 eiwit

VPS24, also known as Charged multivesicular body protein 3 (CHMP3), belongs to the vacuolar sorting protein family and function as chromatin modifying proteins. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Recombinant human VPS24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Charged multivesicular body protein 3, CGI-149, CHMP3, FLJ38114, NEDF

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.2 kDa (242aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

VPS24, Charged multivesicular body protein 3, CGI-149, CHMP3, FLJ38114, NEDF, ATGP1103-10 µg, ATGP1103-20 µg, ATGP1103-50 µg, ATGP1103-100 µg, ATGP1103-250 µg, ATGP1103-500 µg, ATGP1103-1 mg, ATGP1103-10, ATGP1103-20, ATGP1103-50, ATGP1103-100, ATGP1103-250, ATGP1103-500, ATGP1103-1

Muziol T. et al. (2006) Dev. Cell. 10: 821-830.; ; Bache K.G. et al. (2006) Mol. Biol. Cell 17: 2513-2523.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Cell Cycle

NP_057163

Q9Y3E7

Human

MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS