Recombinant humaan VPS25-eiwit

VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Vacuolar protein sorting 25 homolog, Dermal papilla-derived protein 9, DERP9, ELL-associated protein of 20 kDa, EAP20, ESCRT-II complex subunit VPS25

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.3 kDa (200aa) confirmed by MALDI-TOF

VPS25, Vacuolar protein-sorting-associated protein 25, DERP9, EAP20, FAP20, ATGP1447-10 µg, ATGP1447-20 µg, ATGP1447-50 µg, ATGP1447-100 µg, ATGP1447-250 µg, ATGP1447-500 µg, ATGP1447-1 mg, ATGP1447-10, ATGP1447-20, ATGP1447-50, ATGP1447-100, ATGP1447-250, ATGP1447-500, ATGP1447-1

Bailey, S.D. et al. (2010) Diabetes Care 33 (10), 2250-2253; ; Talmud, P.J. et al. (2009) Am. J. Hum. Genet. 85 (5), 628-642;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M Nacl

Signal Transduction

NP_115729

Q9BRG1

Human

MAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF