Recombinant humaan VPS26A-eiwit

VPS26A, also known as vacuolar protein sorting-associated protein 26A, is essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also this protein is required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) . Recombinant human VPS26A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

VPS26 retromer complex component A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26, VPS26, Hbeta58, PEP8A

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

40.6 kDa (350aa) confirmed by MALDI-TOF

VPS26A, Vacuolar protein sorting-associated protein 26A, HB58; Hbeta58; PEP8A; VPS26, ATGP1642-10 µg, ATGP1642-20 µg, ATGP1642-50 µg, ATGP1642-100 µg, ATGP1642-250 µg, ATGP1642-500 µg, ATGP1642-1 mg, ATGP1642-10, ATGP1642-20, ATGP1642-50, ATGP1642-100, ATGP1642-250, ATGP1642-500, ATGP1642-1

Verges M., et al. (2004) Nat. Cell Biol. 6:763-769; ; Gullapalli A., et al. (2006) Mol. Biol. Cell. 17:1228-1238

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Transportation

NP_004887

O75436

Human

MSFLGGF FGPICEIDIV LNDGETRKMA EMKTEDGKVE KHYLFYDGES VSGKVNLAFK QPGKRLEHQG IRIEFVGQIE LFNDKSNTHE FVNLVKELAL PGELTQSRSY DFEFMQVEKP YESYIGANVR LRYFLKVTIV RRLTDLVKEY DLIVHQLATY PDVNNSIKME VGIEDCLHIE FEYNKSKYHL KDVIVGKIYF LLVRIKIQHM ELQLIKKEIT GIGPSTTTET ETIAKYEIMD GAPVKGESIP IRLFLAGYDP TPTMRDVNKK FSVRYFLNLV LVDEEDRRYF KQQEIILWRK APEKLRKQRT NFHQRFESPE SQASAEQPEM