Recombinant humaan VPS28-eiwit

VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E. coli and purified by using conventional chromatography.

VPS28, Vacuolar Protein Sorting 28 acuolar protein sorting 28, Vacuolar Protein Sorting 28, H-Vps28, ESCRT-I complex subunit VPS28

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

25.4 kDa (221aa) confirmed by MALDI-TOF

VPS28, Vacuolar protein sorting 28 homolog, Vacuolar Protein Sorting 28 acuolar protein sorting 28, Vacuolar protein sorting 28 (yeast), Vacuolar Protein Sorting 28, H-Vps28, ESCRT-I complex subunit VPS28, ATGP0400-10 µg, ATGP0400-20 µg, ATGP0400-50 µg, ATGP0400-100 µg, ATGP0400-250 µg, ATGP0400-500 µg, ATGP0400-1 mg, ATGP0400-010, ATGP0400-020, ATGP0400-050, ATGP0400-100, ATGP0400-250, ATGP0400-500, ATGP0400-01M

Woodman P., et al. (2001) J Biol Chem. 276 (15) :11735-42.; ; Bieniasz PD., et al. (2003) J Virol. 77 (8) :4794-804.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Ubiquitination

NP_057292

Q9UK41

Human

MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A