Recombinant humaan VPS29-eiwit

Vacuolar protein sorting 29, also known as VPS29, belongs to a group of genes coding for vacuolar protein sorting (VPS) proteins that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. It is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. Also, VPS29 may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Recombinant human VPS29 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Vacuolar protein sorting-associated protein, DC15, DC7, PEP11, VPS29 retromer complex component, Vesicle protein sorting 29

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.2 kDa (207aa) confirmed by MALDI-TOF

VPS291182aa, Vacuolar protein sorting-associated protein, DC15, DC7, PEP11, ATGP1489-10 µg, ATGP1489-20 µg, ATGP1489-50 µg, ATGP1489-100 µg, ATGP1489-250 µg, ATGP1489-500 µg, ATGP1489-1 mg, ATGP1489-10, ATGP1489-20, ATGP1489-50, ATGP1489-100, ATGP1489-250, ATGP1489-500, ATGP1489-1

Verges M., et al. (2004) Nat. Cell Biol. 6:763-769; ; Damen E., et al. (2006) Biochem. J. 398:399-409

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_057310

Q9UBQ0

Human

MGSSHHHHHH SSGLVPRGSH MGSHMMLVLV LGDLHIPHRC NSLPAKFKKL LVPGKIQHIL CTGNLCTKES YDYLKTLAGD VHIVRGDFDE NLNYPEQKVV TVGQFKIGLI HGHQVIPWGD MASLALLQRQ FDVDILISGH THKFEAFEHE NKFYINPGSA TGAYNALETN IIPSFVLMDI QASTVVTYVY QLIGDDVKVE RIEYKKP