Recombinant humaan VTA1-eiwit

VTA1 (Vps20-associated 1 homolog), also known as LIP5 (LYST-interacting protein 5), DRG1 (Dopamine-responsive protein DRG-1), SBP1 (SKD1 bindingprotein 1), belongs to the VTA1 family. This protein Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Also, VTA1 is known to interact with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3. Recombinant human VTA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

36.4 kDa (331aa) confirmed by MALDI-TOF

VTA1, vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1, ATGP1419-10 µg, ATGP1419-20 µg, ATGP1419-50 µg, ATGP1419-100 µg, ATGP1419-250 µg, ATGP1419-500 µg, ATGP1419-1 mg, ATGP1419-10, ATGP1419-20, ATGP1419-50, ATGP1419-100, ATGP1419-250, ATGP1419-500, ATGP1419-1

Ward D.M., et al. (2005) J. Biol. Chem. 280:10548-10555; ; Shim S., et al. (2008) Mol. Biol. Cell 19:2661-2672

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Transportation

NP_057569.2

Q9NP79

Human

MAALAP LPPLPAQFKS IQHHLRTAQE HDKRDPVVAY YCRLYAMQTG MKIDSKTPEC RKFLSKLMDQ LEALKKQLGD NEAITQEIVG CAHLENYALK MFLYADNEDR AGRFHKNMIK SFYTASLLID VITVFGELTD ENVKHRKYAR WKATYIHNCL KNGETPQAGP VGIEEDNDIE ENEDAGAASL PTQPTQPSSS STYDPSNMPS GNYTGIQIPP GAHAPANTPA EVPHSTGVAS NTIQPTPQTI PAIDPALFNT ISQGDVRLTP EDFARAQKYC KYAGSALQYE DVSTAVQNLQ KALKLLTTGR E