Recombinant humaan VTI1B-eiwit

VTI1B, also known as v-SNARE, mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Recombinant human VTI1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Vesicle transport through interaction with t-SNAREs homolog 1B, v-SNARE, VTI1, VTI1-LIKE, vti1-rp1, VTI1L, VTI2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.3 kDa (231aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

VTI1B, Vesicle transport through interaction with t-SNAREs homolog 1B, v-SNARE, VTI1, VTI1-LIKE, vti1-rp1, VTI1L, VTI2, ATGP1568-10 µg, ATGP1568-20 µg, ATGP1568-50 µg, ATGP1568-100 µg, ATGP1568-250 µg, ATGP1568-500 µg, ATGP1568-1 mg, ATGP1568-10, ATGP1568-20, ATGP1568-50, ATGP1568-100, ATGP1568-250, ATGP1568-500, ATGP1568-1

Fischer von Mollard G., et al. (1998) J. Biol. Chem. 273:2624-2630; ; Daub H., et al. (2008) Mol. Cell. 31:438-448

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_006361

Q9UEU0

Human

MASSAAS SEHFEKLHEI FRGLHEDLQG VPERLLGTAG TEEKKKLIRD FDEKQQEANE TLAEMEEELR YAPLSFRNPM MSKLRNYRKD LAKLHREVRS TPLTATPGGR GDMKYGIYAV ENEHMNRLQS QRAMLLQGTE SLNRATQSIE RSHRIATETD QIGSEIIEEL GEQRDQLERT KSRLVNTSEN LSKSRKILRS MSRKVTTNKL L