Recombinant humaan WDR5-eiwit

WDR5, also designated BMP-2-induced gene 3 kb or BIG-3, belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. This protein is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. WDR5 may play a role in its function of accelerating osteoblast differentiation. Recombinant human WDR5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

WD repeat-containing protein 5, BIG-3, SWD3, WD repeat-containing protein 5, BIG 3, WDR 5, WDR-5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.8 kDa (354aa) confirmed by MALDI-TOF

WDR5, WD repeat-containing protein 5, BIG-3, SWD3, WD repeat-containing protein 5, BIG 3, WDR 5, WDR-5, ATGP0632-10 µg, ATGP0632-20 µg, ATGP0632-50 µg, ATGP0632-100 µg, ATGP0632-250 µg, ATGP0632-500 µg, ATGP0632-1 mg, ATGP0632-10, ATGP0632-20, ATGP0632-50, ATGP0632-100, ATGP0632-250, ATGP0632-500, ATGP0632-1

Yokoyama., et al. (2004) Mol Cell Biol. 24 (13) :5639-49. ; ; Gori F., et al. (2001) J Biol Chem. 276 (49) :46515-22. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Epigenomics (Transcription & Translation)

NP_438172.1

P61964

Human

MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH TDVVISTACH PTENIIASAA LENDKTIKLW KSDC