Recombinant humaan WIBG-eiwit

WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC (exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain. Recombinant human WIBG protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Partner of Y14 and mago, PYM, Partner of Y14 and mago

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.7 kDa (212aa) confirmed by MALDI-TOF

WIBG, Partner of Y14 and mago, PYM, Partner of Y14 and mago, ATGP0635-10 µg, ATGP0635-20 µg, ATGP0635-50 µg, ATGP0635-100 µg, ATGP0635-250 µg, ATGP0635-500 µg, ATGP0635-1 mg, ATGP0635-10, ATGP0635-20, ATGP0635-50, ATGP0635-100, ATGP0635-250, ATGP0635-500, ATGP0635-1

Bono F, . et al. (2004) EMBO Rep. 5 (3) :304-10.; ; Gehring NH, et al. (2009) Cell. 137 (3) :536-48.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl ,0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_115721.2

Q9BRP8

Human