Recombinant humaan WIF-1-eiwit

Wnt inhibitory factor 1, also known as Wif-1, is a secreted protein that binds to Wnt proteins involved in the embryonic development and cancer, and inhibits their activity. Wif-1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF) -like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. Recombinant human Wif-1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Wnt inhibitory factor 1, Wnt inhibitory factor 1, WIF1, Wnt inhibitory factor 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.2 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

39.5 kDa (360aa)

WIF1, Wnt inhibitory factor 1, Wnt inhibitory factor 1, WIF1, Wnt inhibitory factor 1, ATGP0305-10 µg, ATGP0305-20 µg, ATGP0305-50 µg, ATGP0305-100 µg, ATGP0305-250 µg, ATGP0305-500 µg, ATGP0305-1 mg, ATGP0305-10, ATGP0305-20, ATGP0305-50, ATGP0305-100, ATGP0305-250, ATGP0305-500, ATGP0305-1

Wissmann C. et al. (2003) J. Pathol. 201:204-12; ; Cebrat M. et al. (2004) Cancer Lett. 206:107-13;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF, 1mM DTT

Cancer

AAH18037.1

Q9Y5W5

Human

GPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLG TVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL QTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCV TPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNK CQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIW