Recombinant humaan WKK-2/PTPN11-eiwit

PTPN11, also known as tyrosine-protein phosphatase non-receptor type 11 isoform 2, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. It participates in signal transduction from the cell surface to the nucleus downstream of various receptor and cytosolic protein tyrosine kinases. This protein deficient cells have poor mobility because excessive phosphorylation of FAK and other proteins interferes with the rotation of cell attachment points in the focal adhesion complex. Recombinant human PTPN11, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Protein tyrosine phosphatase non-receptor type 11, NS1, Noonan syndrome 1, SH2 domain-containing protein tyrosine phosphatase 2, SHP-2, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, PTP2C, SHP2, BPTP3, SH-PTP2

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

53.9 kDa (469aa)

PTPN11, Tyrosine-protein phosphatase non-receptor type 11 isoform 2, PTPN11, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, ATGP3714-10 µg, ATGP3714-20 µg, ATGP3714-50 µg, ATGP3714-100 µg, ATGP3714-250 µg, ATGP3714-500 µg, ATGP3714-1 mg, ATGP3714-10, ATGP3714-20, ATGP3714-50, ATGP3714-100, ATGP3714-250, ATGP3714-500, ATGP3714-1

Clemmons, D.R., et al. (2005) Mol. Endocrinol. 19:1-11.; ; Maroun, C.R., et al. (2000) Mol. Cell. Biol. 20:8513-8525.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_542168.1

Q06124

Human

Ala

MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCR