Recombinant humaan WKK-2/PTPN11-eiwit

SHP-2, also known as, tyrosine-protein phospatase non-receptor type 11 (PTPN11), is a member of the protein tyrosine phosphatase (PTP) family containing two Src homology 2 domains. This protein dephosphorylates tyrosine residues in proteins. It plays a stimulatory role in activation of the Erk/MAP kinase pathway by receptor tyrosine kinase signaling. Mutations in this protein are a cause of Noonan syndrome as well as acute myeloid leukemia. Recombinant human SHP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 400 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Protein tyrosine phosphatase non-receptor type 11, NS1, Noonan syndrome 1, SH2 domain-containing protein tyrosine phosphatase 2, SHP-2, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, PTP2C, SHP2, BPTP3, SH-PTP2

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 400 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

69.1kDa (602aa)

, ATGP3943-10 µg, ATGP3943-20 µg, ATGP3943-50 µg, ATGP3943-100 µg, ATGP3943-250 µg, ATGP3943-500 µg, ATGP3943-1 mg, ATGP3943-10, ATGP3943-20, ATGP3943-50, ATGP3943-100, ATGP3943-250, ATGP3943-500, ATGP3943-1

Giorgia R, et al, (2018) Cell Rep. 23:39-49. ; ; Chitranshi N., et al, (2017) Neuroscience. 364:175-189. ; ; Batth TS, et al, (2018) Cell Rep. 22:2784-2796. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Signal Transduction, Enzymes & Proteases, Epigenetics and Nuclear Signaling

NP_002825

Q06124

Human

Ala

MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RIEEEQKSKR KGHEYTNIKY SLADQTSGDQ SPLPPCTPTP PCAEMREDSA RVYENVGLMQ QQKSFR