Recombinant humaan XAB1-eiwit

XAB1, as known as GPN1, belongs to the GPN-loop GTPase family. Small GTPases share a biochemical mechanism and act as binary molecular switches. One important function of small GTPases in the cell is nucleocytoplasmic transport of both proteins and RNA. This protein may play a role in DNA repair and may function in activation of transcription. And it forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Recombinant human XAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

XPA binding protein 1 GTPase, XPA binding protein 1, GTPase, GPN1, ATPBD1A, MBDIN, NTPBP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44.3 kDa (398aa) confirmed by MALDI-TOF

XAB1, XPA binding protein 1 GTPase, XPA binding protein 1, GTPase, GPN1, ATPBD1A, MBDIN, NTPBP, ATGP1601-10 µg, ATGP1601-20 µg, ATGP1601-50 µg, ATGP1601-100 µg, ATGP1601-250 µg, ATGP1601-500 µg, ATGP1601-1 mg, ATGP1601-10, ATGP1601-20, ATGP1601-50, ATGP1601-100, ATGP1601-250, ATGP1601-500, ATGP1601-1

Carre C, et al. (2011) Mol Cell Biol. 31 (19) :3953-62.; ; Forget D, et al. (2010) Mol Cell Proteomics. 9 (12) :2827-39.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Epigenomics (Transcription & Translation)

NP_009197

Q9HCN4

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAASAA AAELQASGGP RHPVCLLVLG MAGSGKTTFV QRLTGHLHAQ GTPPYVINLD PAVHEVPFPA NIDIRDTVKY KEVMKQYGLG PNGGIVTSLN LFATRFDQVM KFIEKAQNMS KYVLIDTPGQ IEVFTWSASG TIITEALASS FPTVVIYVMD TSRSTNPVTF MSNMLYACSI LYKTKLPFIV VMNKTDIIDH SFAVEWMQDF EAFQDALNQE TTYVSNLTRS MSLVLDEFYS SLRVVGVSAV LGTGLDELFV QVTSAAEEYE REYRPEYERL KKSLANAESQ QQREQLERLR KDMGSVALDA GTAKDSLSPV LHPSDLILTR GTLDEEDEEA DSDTDDIDHR VTEESHEEPA FQNFMQESMA QYWKRNNK