Recombinant humaan XAF1-eiwit

XAF1 is a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human XAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

XIAP associated factor 1 isoform 1, XIAP associated factor 1 isoform 1, BIRC4BP, HSXIAPAF1, XIAPAF1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.6 kDa (161aa) confirmed by MALDI-TOF

XAF1, XIAP associated factor 1 isoform 1, XIAP associated factor 1 isoform 1, BIRC4BP, HSXIAPAF1, XIAPAF1, ATGP2678-10 µg, ATGP2678-20 µg, ATGP2678-50 µg, ATGP2678-100 µg, ATGP2678-250 µg, ATGP2678-500 µg, ATGP2678-1 mg, ATGP2678-10, ATGP2678-20, ATGP2678-50, ATGP2678-100, ATGP2678-250, ATGP2678-500, ATGP2678-1

Liston P., et al (2001) . Nat. Cell Biol. 3:128-133; ; Leaman D.W., et al (2002) . J. Biol. Chem. 277:28504-28511;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl

Apoptosis

NP_059993.2

Q6GPH4

Human

MEGD FSVCRNCKRH VVSANFTLHE AYCLRFLVLC PECEEPVPKE TMEEHCKLEH QQVGCTMCQQ SMQKSSLEFH KANECQERPV ECKFCKLDMQ LSKLELHESY CGSRTELCQG CGQFIMHRML A