Recombinant humaan XAGE1A-eiwit

G antigen family D member 2, also known as XAGE1A, is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Recombinant human XAGE1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

G antigen family D member 2, CT12.1, CT12.1A, CTP9, GAGED2, XAGE1, XAGE1B, XAGE1C, XAGE1D, XAGE1E

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

11.5 kDa (104aa) confirmed by MALDI-TOF

XAGE1A, ATGP1763-10 µg, ATGP1763-20 µg, ATGP1763-50 µg, ATGP1763-100 µg, ATGP1763-250 µg, ATGP1763-500 µg, ATGP1763-1 mg, ATGP1763-10, ATGP1763-20, ATGP1763-50, ATGP1763-100, ATGP1763-250, ATGP1763-500, ATGP1763-1

Egland K.A., et al. (2002) Mol. Cancer Ther. 1:441-450; ; Sato S., et al. (2007) Cancer Immun. 7:5-5

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 30% glycerol, 0.1mM PMSF

Cancer

NP_001091061.2

Q9HD64

Human

MESPKKK NQQLKVGILH LGSRQKKIRI QLRSQCATWK VICKSCISQT PGINLDLGSG VKVKIIPKEE HCKMPEAGEE QPQV