Recombinant humaan YARS-eiwit

Tyrosyl-tRNA synthetase, also known as YARS, belongs to the class I tRNA synthetase family. YARS is essential enzyme that catalyze the aminoacylation of tRNATrp with tryptophan, an essential function of the cell's protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma. Recombinant human YARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tyrosyl-tRNA synthetase, CMTDIC, TYRRS, YRS, YTS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

61.3 kDa (548aa)

YARS, Tyrosyl-tRNA synthetase, CMTDIC, TYRRS, YRS, YTS, ATGP1055-10 µg, ATGP1055-20 µg, ATGP1055-50 µg, ATGP1055-100 µg, ATGP1055-250 µg, ATGP1055-500 µg, ATGP1055-1 mg, ATGP1055-10, ATGP1055-20, ATGP1055-50, ATGP1055-100, ATGP1055-250, ATGP1055-500, ATGP1055-1

Kise Y., et al. (2004) Nat Struct Mol Biol. 11:149-156.; ; Wakasugi K., et al. (2002) Proc Natl Acad Sci . 99:173-177.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_003671.1

P54577

Human

MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS