Recombinant humaan YARS2-eiwit

YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA (Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA (Tyr) . Recombinant human YARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Tyrosyl-tRNA synthetase mitochondrial precursor, Tyrosyl-tRNA synthetase, mitochondrial precursor, CGI-04, FLJ13995, MLASA2, MT-TYRRS, TYRRS

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.6 kDa (482aa)

YARS2, Tyrosyl-tRNA synthetase mitochondrial precursor, Tyrosyl-tRNA synthetase, mitochondrial precursor, CGI-04, FLJ13995, MLASA2, MT-TYRRS, TYRRS, ATGP1554-10 µg, ATGP1554-20 µg, ATGP1554-50 µg, ATGP1554-100 µg, ATGP1554-250 µg, ATGP1554-500 µg, ATGP1554-1 mg, ATGP1554-10, ATGP1554-20, ATGP1554-50, ATGP1554-100, ATGP1554-250, ATGP1554-500, ATGP1554-1

Bonnefond L., et al. (2005) Biochemistry. 44:4805-4816; ; Riley L.G., et al. (2010) Am. J. Hum. Genet. 87:52-59

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Enzymes & Proteases

NP_001035526

Q9Y2Z4

Human

TLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL