Recombinant humaan YBX1-eiwit

YBX1, also known as Y box binding protein 1, associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation. This protein is a potential drug target in cancer therapy. It helps the replication of adenovirus type 5, a commonly used vector in gene therapy. Thus, YBX1 can cause an oncolytic effect in YBX1 positive cancer cells treated with adenoviruses. Recombinant human YBX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Nuclease-sensitive element-binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.3 kDa (347aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

YBX1, Nuclease-sensitive element-binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1, ATGP2859-10 µg, ATGP2859-20 µg, ATGP2859-50 µg, ATGP2859-100 µg, ATGP2859-250 µg, ATGP2859-500 µg, ATGP2859-1 mg, ATGP2859-10, ATGP2859-20, ATGP2859-50, ATGP2859-100, ATGP2859-250, ATGP2859-500, ATGP2859-1

Shen Q. et al. (2006) J Cell Physiol. 207:775-783.; ; Chernukhin IV. et al. (2000) J Biol Chem. 275:29915-29921

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Epigenomics (Transcription & Translation)

NP_004550.2

P67809

Human

MSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE