Recombinant humaan YEATS4-eiwit

YEATS4 has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Recombinant human YEATS4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

YEATS domain-containing protein 4, 4930573H17Rik, B230215M10Rik, GAS41, NuBI-1, YAF9, YEATS domain containing 4, Glioma-amplified sequence 41

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.9 kDa (250aa) confirmed by MALDI-TOF

YEATS4, YEATS domain-containing protein 4, 4930573H17Rik, B230215M10Rik, GAS41, NuBI-1, YAF9, YEATS domain containing 4, Glioma-amplified sequence 41, ATGP1365-10 µg, ATGP1365-20 µg, ATGP1365-50 µg, ATGP1365-100 µg, ATGP1365-250 µg, ATGP1365-500 µg, ATGP1365-1 mg, ATGP1365-10, ATGP1365-20, ATGP1365-50, ATGP1365-100, ATGP1365-250, ATGP1365-500, ATGP1365-1

Harborth J. et al. (2000) J. Biol. Chem. 275:31979-31985.; ; Debernardi S. et al. (2002) Blood. 99: 275-81.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50% glycerol, 2mM DTT, 0.2M NaCl, 0.1mM PMSF, 50 mM Imidazole

Epigenomics (Transcription & Translation)

NP_006521

O95619

Human

MFKRMAE FGPDSGGRVK GVTIVKPIVY GNVARYFGKK REEDGHTHQW TVYVKPYRNE DMSAYVKKIQ FKLHESYGNP LRVVTKPPYE ITETGWGEFE IIIKIFFIDP NERPVTLYHL LKLFQSDTNA MLGKKTVVSE FYDEMIFQDP TAMMQQLLTT SRQLTLGAYK HETEFAELEV KTREKLEAAK KKTSFEIAEL KERLKASRET INCLKNEIRK LEEDDQAKDI