Recombinant humaan YOD1-eiwit

YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin thioesterase OTu1, ubiquitin thioesterase OTu1, DuBA8, OTuD2, PRO0907, RP11-164O23.1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40.7 kDa (371aa) confirmed by MALDI-TOF

YOD1, ubiquitin thioesterase OTu1, ubiquitin thioesterase OTu1, DuBA8, OTuD2, PRO0907, RP11-164O23.1, ATGP2019-10 µg, ATGP2019-20 µg, ATGP2019-50 µg, ATGP2019-100 µg, ATGP2019-250 µg, ATGP2019-500 µg, ATGP2019-1 mg, ATGP2019-10, ATGP2019-20, ATGP2019-50, ATGP2019-100, ATGP2019-250, ATGP2019-500, ATGP2019-1

Ernst, R., et al. (2009) Mol. Cell 36 (1), 28-38

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Signal Transduction

NP_061036.3

Q5VVQ6

Human

MFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V