Recombinant humaan YY1-eiwit

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. This protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Recombinant human YY1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

YY1 transcription factor, DELTA, INO80S, NF-E1, uCRBP, YIN-YANG-1, YIN YANG 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

47.1 kDa (437aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

YY1, YY1 transcription factor, DELTA, INO80S, NF-E1, uCRBP, YIN-YANG-1, YIN YANG 1, ATGP2855-10 µg, ATGP2855-20 µg, ATGP2855-50 µg, ATGP2855-100 µg, ATGP2855-250 µg, ATGP2855-500 µg, ATGP2855-1 mg, ATGP2855-10, ATGP2855-20, ATGP2855-50, ATGP2855-100, ATGP2855-250, ATGP2855-500, ATGP2855-1

Cai Y., et al (2007) Nat. Struct. Mol. Biol. 14:872-874 ; ; Wu S., et al (2007) Nat. Struct. Mol. Biol. 14:1165-1172

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_003394.1

P25490

Human

MASGDTL YIATDGSEMP AEIVELHEIE VETIPVETIE TTVVGEEEEE DDDDEDGGGG DHGGGGGHGH AGHHHHHHHH HHHPPMIALQ PLVTDDPTQV HHHQEVILVQ TREEVVGGDD SDGLRAEDGF EDQILIPVPA PAGGDDDYIE QTLVTVAAAG KSGGGGSSSS GGGRVKKGGG KKSGKKSYLS GGAGAAGGGG ADPGNKKWEQ KQVQIKTLEG EFSVTMWSSD EKKDIDHETV VEEQIIGENS PPDYSEYMTG KKLPPGGIPG IDLSDPKQLA EFARMKPRKI KEDDAPRTIA CPHKGCTKMF RDNSAMRKHL HTHGPRVHVC AECGKAFVES SKLKRHQLVH TGEKPFQCTF EGCGKRFSLD FNLRTHVRIH TGDRPYVCPF DGCNKKFAQS TNLKSHILTH AKAKNNQ